Skip to main content
SpringerLink
Log in

A stepwise method for the isolation of endothelial cells and smooth muscle cells from individual canine coronary arteries

  • Reports
  • Published:
In Vitro Cellular & Developmental Biology - Animal Aims and scope Submit manuscript

Summary

Methods for the stepwise isolation of endothelial cells and smooth muscle cells from individual canine coronary arteries are described. Both cell types can be isolated in pure culture with high yields. Dogs are a common species used in the study of atherosclerosis and coronary artery disease. Capacity to isolate endothelial cells and smooth muscle cells from individual canine coronary arteries should prove useful in the study of coronary artery disease.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Albassam, M. A.; Metz, A. L.; Potoczak, R. E.; Gallagher, K. P.; Haleen, S.; Hallak, H.; McGuire, E. J. Studies on coronary arteriopathy in dogs following administration of CI-1020, an endothelin A receptor antagonist. Toxicol. Pathol. 29:277–284; 2001.

    Article  PubMed  CAS  Google Scholar 

  • Axel, D. I.; Brehm, B. R.; Wolburg-Buchholz, K.; Betz, E. L.; Koveker, G.; Karsch, K. R. Induction of cell-rich and lipid-rich plaques in a transfilter coculture system with human vascular cells. J. Vasc. Res. 33:327–339; 1996.

    Article  PubMed  CAS  Google Scholar 

  • Chamley-Campbell, J. H.; Campbell, G. R.; Ross, R. Phenotype-dependent response of cultured aortic smooth muscle to serum mitogens. J. Cell Biol. 89:379–383; 1981.

    Article  PubMed  CAS  Google Scholar 

  • Grafe, M.; Graf, K.; Auch-Schwelk, W.; Terbeek, D.; Hertel, H.; Fleck, E. Cultivation and characterization of micro- and macrovascular endothelial cells from the human heart. Eur. Heart J. 14 (Suppl 1): 74–81; 1993.

    PubMed  Google Scholar 

  • Gu, J. W.; Adair, T. H. Hypoxia-induced expression of VEGF is reversible in myocardial vascular smooth muscle cells. Am. J. Physiol. 273:H628-H633; 1997.

    PubMed  CAS  Google Scholar 

  • Jaffe, E. A.; Nachman, R. L.; Becker, C. G.; Minick, C. R. Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J. Clin. Invest. 52:2745–2756; 1973.

    Article  PubMed  CAS  Google Scholar 

  • Ogawa, K.; Miki, M.; Hirata, M.; Funahara, Y.; Kitaguchi, H. Plasminogen activator activity of cultured endothelial cells derived from canine coronary vessel and human umbilical artery and vein. Thrombo. Res. 37:411–422; 1985.

    Article  CAS  Google Scholar 

  • Roth, D. R.; Axel, D. I.; Betz, E. L. In vitro model of the inner parts of a vessel wall with cultured human vascular cells. Coron. Artery Dis. 4:283–291; 1993.

    Article  PubMed  CAS  Google Scholar 

  • Stephan-Gueldner, M.; Inomata, A. Coronary arterial lesions induced by endothelin antagonists. Toxicol Lett. 112/113:531–535; 2000.

    Article  Google Scholar 

  • Varani, J.; Dame, M. K.; Gibbs, D. F.; Taylor, C. G.; Weinberg, J. M.; Shayevitz, J.; Ward, P. A. Human umbilical vein endothelial cell killing by activated neutrophils. Loss of sensitivity to injury is accompanied by decreased iron content during in vitro culture and is restored with exogenous iron. Lab. Invest. 66:708–714; 1992.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pathology, University of Michigan Medical School, 4224 Medical Science I Building, 1301 Catherine Street, 48109-0602, Ann Arbor, Michigan

    Michael K. Dame, Hedwig S. Murphy & James Varani

  2. Pfizer Global Research and Development, Pfizer, 2800 Plymouth, 48105, Ann Arbor, Michigan

    Xinwen Yu, Rosario Garrido, Walter Bobrowski, J. Eric McDuffie & Mudher Albassam

  3. Pathology and Laboratory Medicine, Veterans Administration Ann Arbor Health System, 48105-2303, Ann Arbor, Michigan

    Hedwig S. Murphy

Authors
  1. Michael K. Dame
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xinwen Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rosario Garrido
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Walter Bobrowski
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. Eric McDuffie
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Hedwig S. Murphy
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Mudher Albassam
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. James Varani
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Michael K. Dame.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dame, M.K., Yu, X., Garrido, R. et al. A stepwise method for the isolation of endothelial cells and smooth muscle cells from individual canine coronary arteries. In Vitro Cell.Dev.Biol.-Animal 39, 402–406 (2003). https://doi.org/10.1290/1543-706X(2003)039<0402:ASMFTI>2.0.CO;2

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1290/1543-706X(2003)039<0402:ASMFTI>2.0.CO;2

Keywords

Search

Navigation